Prohibitin 2 anticorps (AA 1-299)

N° du produit ABIN7599239

L’anticorps Souris Monoclonal anti-Prohibitin 2 a été validé pour WB, IHC et FACS. Il convient pour détecter Prohibitin 2 dans des échantillons de Humain, Souris et Rat.

Aperçu rapide pour Prohibitin 2 anticorps (AA 1-299) (ABIN7599239)

Antigène: Prohibitin 2 (PHB2)

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Prohibitin 2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Clone: 2B5

Détail du produit anti-Prohibitin 2 anticorps

Épitope: AA 1-299

Fonction: Anti-REA/PHB2 Antibody Picoband® (monoclonal, 2B5)

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-REA/PHB2 Antibody Picoband® (monoclonal, 2B5) (ABIN7599239). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human REA/PHB2 recombinant protein (Position: M1-K299).

Isotype: IgG2b

Information d'application

Indications d'application: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Merkwirth, C., Dargazanli, S., Tatsuta, T., Geimer, S., Lower, B., Wunderlich, F. T., von Kleist-Retzow, J.-C., Waisman, A., Westermann, B., Langer, T. Prohibitins control cell proliferation and apoptosis by regulating OPA1-dependent cristae morphogenesis in mitochondria. Genes Dev. 22: 476-488, 2008. 2. Montano, M. M., Ekena, K., Delage-Mourroux, R., Chang, W., Martini, P., Katzenellenbogen, B. S. An estrogen receptor-selective coregulator that potentiates the effectiveness of antiestrogens and represses the activity of estrogens. Proc. Nat. Acad. Sci. 96: 6947-6952, 1999. 3. Mussi, P., Liao, L., Park, S.-E., Ciana, P., Maggi, A., Katzenellenbogen, B. S., Xu, J., O'Malley, B. W. Haploinsufficiency of the corepressor of estrogen receptor activity (REA) enhances estrogen receptor function in the mammary gland. Proc. Nat. Acad. Sci. 103: 16716-16721, 2006.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur Prohibitin 2

Antigène: Prohibitin 2 (PHB2)

Autre désignation: PHB2

Sujet:

Synonyms: Interleukin-32, IL-32, Natural killer cells protein 4, Tumor necrosis factor alpha-inducing factor, IL32, NK4, TAIF

Tissue Specificity: Expressed in all tissues, but more abundant in heart, brain and skeletal muscle.

Background: PHB2 (Prohibitin 2), also called Repressor of Estrogen Receptor Activity (REA), is a protein that in humans is encoded by the PHB2 gene. The International Radiation Hybrid Mapping Consortium mapped the PHB2 gene to chromosome 12. Montano et al. (1999) showed that REA enhanced the potency of a dominant-negative ER-alpha mutant and antiestrogens as suppressors of ER-alpha activity in Chinese hamster ovary cells. When coexpressed with wildtype ER-alpha or ER-beta (ESR2), REA suppressed activation of a reporter gene in a dose-dependent manner. REA had no effect on reporter activity in the absence of liganded ER, and it had no effect on the transcriptional activities of other hormone receptors. Mutation analysis showed that an N-terminal domain and a central domain of REA were required for its repressor activity.

Poids moléculaire: 32 kDa

ID gène: 11331

UniProt: Q99623

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling